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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAVS; VISA Recombinant antibody (81910-1-RR)

    WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    WB analysis of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    WB result of MAVS; VISA antibody (81910-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAVS; VISA Recombinant antibody (81910-1-RR)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAVS; VISA Recombinant antibody (81910-1-RR)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81910-1-RR

    Affinity and Kinetic Characterization of 81910-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is 0.338 nM.

  • Featured Product
  • KD/KO Validated

MAVS; VISA Recombinant monoclonal antibody, PBS Only

MAVS; VISA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 81910-1-PBS
Clone No.7F14

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MAVS, 7F14, Cardif, IPS 1, IPS1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81910-1-PBS targets MAVS; VISA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5655

Product name: Recombinant human MAVS; VISA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC044952

Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS

相同性解析による交差性が予測される生物種
Full Name mitochondrial antiviral signaling protein
Calculated molecular weight 57 kDa
Observed molecular weight75 kDa
GenBank accession numberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

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