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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAVS; VISA Recombinant antibody, PBS Only

MAVS; VISA Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 81910-1-PBS

Synonyms

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, VISA

Validation Data Gallery

Applications:
  • WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

81910-1-PBS targets MAVS; VISA in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAVS; VISA fusion protein Ag5655 相同性解析による交差性が予測される生物種
Full Name mitochondrial antiviral signaling protein
Calculated molecular weight 57 kDa
Observed molecular weight75 kDa
GenBank accession numberBC044952
Gene symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.