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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis of HeLa using 66911-1-Ig (same clone as 66911-1-PBS)

    WB result of MAVS; VISA antibody (66911-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis of L02 using 66911-1-Ig (same clone as 66911-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis of Jurkat using 66911-1-Ig (same clone as 66911-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAVS; VISA Monoclonal antibody (66911-1-Ig)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50509-3

    Cytometric bead array standard curve of MP50509-3

    Cytometric bead array standard curve of MP50509-3, MAVS; VISA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66911-1-PBS. Detection antibody: 66911-4-PBS. Standard:Ag5949. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO Validated

MAVS; VISA Monoclonal antibody, PBS Only (Capture)

MAVS; VISA Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66911-1-PBS
Clone No.1A8E9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

MAVS, 1A8E9, Cardif, IPS 1, IPS1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66911-1-PBS targets MAVS; VISA as part of a matched antibody pair:

MP50509-3: 66911-1-PBS capture and 66911-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5949

Product name: Recombinant human MAVS; VISA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-347 aa of BC044952

Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS

相同性解析による交差性が予測される生物種
Full Name mitochondrial antiviral signaling protein
Calculated molecular weight 57 kDa
Observed molecular weight50-55 kDa, 70-75 kDa
GenBank accession numberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
RRIDAB_2882238
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

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