Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 15952-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15952-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15952-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15952-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15952-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15952-1-AP
IP result of anti-MAT2B (IP:15952-1-AP, 4ug; Detection:15952-1-AP 1:500) with HepG2 cells lysate 1000ug.
IHC staining of human lung using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse lung using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15952-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 15952-1-AP
Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 15952-1-AP
Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MAT2B Polyclonal antibody

MAT2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15952-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Nbla02999, MSTP045, MATIIbeta, MAT II beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human lung tissue, mouse lung tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 23 publications below
IHC	See 1 publications below

Product Information

15952-1-AP targets MAT2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8649 Product name: Recombinant human MAT2B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-323 aa of BC005218 Sequence: MPEMPEDMEQEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNTQLDCSKLETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH 相同性解析による交差性が予測される生物種
Full Name	methionine adenosyltransferase II, beta
Calculated molecular weight	36 kDa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC005218
Gene Symbol	MAT2B
Gene ID (NCBI)	27430
RRID	AB_10637268
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZL9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for MAT2B antibody 15952-1-AP	Download protocol
IHC protocol for MAT2B antibody 15952-1-AP	Download protocol
IP protocol for MAT2B antibody 15952-1-AP	Download protocol
WB protocol for MAT2B antibody 15952-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
human		Ann N Y Acad Sci PMID: 29722030 Authors - Holly M Brown-Borg View Article
rat	WB	J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. Authors - Shu Yang View Article
pig	WB	J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Authors - Yimin Jia View Article
chicken	WB	Sci Rep In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications. Authors - Yun Hu View Article
rat	WB	J Nutr Biochem Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats. Authors - Nannan Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 15952-1-AP

WB analysis of Jurkat using 15952-1-AP

WB analysis of HEK-293 using 15952-1-AP

WB analysis of mouse testis using 15952-1-AP

WB analysis of mouse brain using 15952-1-AP

IP experiment of HepG2 using 15952-1-AP

IHC staining of human lung using 15952-1-AP

IHC staining of human lung using 15952-1-AP

IHC staining of mouse lung using 15952-1-AP

IHC staining of mouse lung using 15952-1-AP

IHC staining of human kidney using 15952-1-AP

IHC staining of human kidney using 15952-1-AP

IF Staining of HepG2 using 15952-1-AP

IF Staining of HepG2 using 15952-1-AP

MAT2B Polyclonal antibody

MAT2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

PMID: 29722030

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications.

Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.