Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
13305-1-AP targets MAST1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4126 Product name: Recombinant human MAST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 494-769 aa of BC027985 Sequence: TNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASS 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule associated serine/threonine kinase 1 |
| Calculated molecular weight | 1570 aa, 171 kDa |
| Observed molecular weight | 171 kDa |
| GenBank accession number | BC027985 |
| Gene Symbol | MAST1 |
| Gene ID (NCBI) | 22983 |
| RRID | AB_10639043 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2H9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MAST1 antibody 13305-1-AP | Download protocol |
| WB protocol for MAST1 antibody 13305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.
| ||
Theranostics CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells |
