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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MARVELD2 Recombinant antibody (86188-1-RR)

    WB analysis of HeLa using 86188-1-RR (same clone as 86188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86188-1-RR (MARVELD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86188-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MARVELD2 Recombinant antibody (86188-1-RR)

    IF Staining of MCF-7 using 86188-1-RR (same clone as 86188-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MARVELD2 antibody (86188-1-RR, Clone: 250774G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86188-1-PBS in a different storage buffer formulation.

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MARVELD2 Recombinant antibody, PBS Only

MARVELD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86188-1-PBS
Clone No.250774G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DFNB49, MARVD2, MARVEL domain containing 2, MARVEL domain-containing protein 2, MRVLDC2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86188-1-PBS targets MARVELD2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MARVELD2 fusion protein Ag4446 相同性解析による交差性が予測される生物種
Full Name MARVEL domain containing 2
Calculated molecular weight 558 aa, 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC033689
Gene Symbol MARVELD2
Gene ID (NCBI) 153562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N4S9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.