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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using MARCH1 Recombinant antibody (85308-2-RR)

    WB analysis of Daudi using 85308-2-RR (same clone as 85308-2-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MARCH1 Recombinant antibody (85308-2-RR)

    WB analysis using 85308-2-RR (same clone as 85308-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85308-2-RR

    Affinity and Kinetic Characterization of 85308-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

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MARCH1 Recombinant antibody, PBS Only

MARCH1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85308-2-PBS
Clone No.242143G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85308-2-PBS targets MARCH1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MARCH1 fusion protein Ag20231 相同性解析による交差性が予測される生物種
Full Name membrane-associated ring finger (C3HC4) 1
Calculated molecular weight 289 aa, 32 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC153124
Gene Symbol MARCHF1
Gene ID (NCBI) 55016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TCQ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).