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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPKBP1 Polyclonal antibody

MAPKBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17788-1-AP

Synonyms

JNK binding protein 1, JNKBP 1, JNKBP1, KIAA0596, MAPKBP1

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 17788-1-AP

    WB analysis of mouse liver using 17788-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17788-1-AP (MAPKBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 17788-1-AP

    WB analysis of rat liver using 17788-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 17788-1-AP (MAPKBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 17788-1-AP

    IHC staining of human pancreas using 17788-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17788-1-AP (MAPKBP1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MAPKBP1 antibody included
  • IHC staining of human pancreas using 17788-1-AP

    IHC staining of human pancreas using 17788-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17788-1-AP (MAPKBP1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MAPKBP1 antibody included
  • IF Staining of HepG2 using 17788-1-AP

    IF Staining of HepG2 using 17788-1-AP

    Immunofluorescent analysis of HepG2 cells, using MAPKBP1 antibody 17788-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MAPKBP1 Ready-To-Use IHC Kit. MAPKBP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17788-1-AP targets MAPKBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKBP1 fusion protein Ag11883 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase binding protein 1
Calculated molecular weight 1015 aa, 109 kDa, 164 kDa
Observed molecular weight 200-230 kDa
GenBank accession numberBC036660
Gene symbol MAPKBP1
Gene ID (NCBI) 23005
RRIDAB_2235097
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPKBP1 antibody 17788-1-APDownload protocol
IHC protocol for MAPKBP1 antibody 17788-1-APDownload protocol
IF protocol for MAPKBP1 antibody 17788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols