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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of Ramos using 60252-1-Ig (same clone as 60252-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of HepG2 using 60252-1-Ig (same clone as 60252-1-PBS)

    WB result of MAPK13 antibody (60252-1-Ig, 1:2000) with si-Control and si-MAPK13 transfected HepG2 cells. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of HEK-293 using 60252-1-Ig (same clone as 60252-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using MAPK13 Monoclonal antibody (60252-1-Ig)

    WB analysis of Daudi using 60252-1-Ig (same clone as 60252-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAPK13 Monoclonal antibody (60252-1-Ig)

    IHC staining of human pancreas cancer using 60252-1-Ig (same clone as 60252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAPK13 Monoclonal antibody (60252-1-Ig)

    IHC staining of human pancreas cancer using 60252-1-Ig (same clone as 60252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MAPK13 Monoclonal antibody, PBS Only

MAPK13 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60252-1-PBS
Clone No.1B12A6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60252-1-PBS targets MAPK13 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAPK13 fusion protein Ag21240 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 13
Calculated molecular weight 38 kDa
Observed molecular weight38-42 kDa
GenBank accession numberBC000433
Gene Symbol MAPK13
Gene ID (NCBI) 5603
RRIDAB_2881373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15264
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13, SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.