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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

SAPK4 Polyclonal antibody

SAPK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10217-1-AP

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

Validation Data Gallery

Applications:
  • WB analysis using 10217-1-AP

    WB analysis using 10217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10217-1-AP (SAPK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10217-1-AP

    IHC staining of human pancreas cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • IHC staining of human pancreas cancer using 10217-1-AP

    IHC staining of human pancreas cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • IHC staining of human liver cancer using 10217-1-AP

    IHC staining of human liver cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • IHC staining of human liver cancer using 10217-1-AP

    IHC staining of human liver cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • IHC staining of human liver cancer using 10217-1-AP

    IHC staining of human liver cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • IHC staining of human liver cancer using 10217-1-AP

    IHC staining of human liver cancer using 10217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included

Tested Applications

Positive WB detected inU-937 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK13 Ready-To-Use IHC Kit. MAPK13 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10217-1-AP targets SAPK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity hamster, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAPK4 fusion protein Ag0255 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 13
Calculated molecular weight 38 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC000433
Gene symbol MAPK13
Gene ID (NCBI) 5603
RRIDAB_2877727
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SAPK4 antibody 10217-1-APDownload protocol
IHC protocol for SAPK4 antibody 10217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Sci Rep

Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.

Authors - Lingling Han
View Article
humanWB,IHC

Tumour Biol

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

Authors - Shutao Zheng
  • KD Validated
View Article
ratWB

BMC Endocr Disord

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

Authors - Shuman Wang
View Article