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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAPK10 Recombinant antibody (84875-5-RR)

    IF Staining of HepG2 using 84875-5-RR (same clone as 84875-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAPK10 antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MAPK10 Recombinant antibody (84875-5-RR)

    FC experiment of HeLa using 84875-5-RR (same clone as 84875-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84875-5-RR

    Affinity and Kinetic Characterization of 84875-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.

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MAPK10 Recombinant antibody, PBS Only

MAPK10 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84875-5-PBS
Clone No.242364A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

JNK, JNK1/2/3, JNK1, EC:2.7.11.24, c-Jun N-terminal kinase 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84875-5-PBS targets MAPK10 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAPK10 fusion protein Ag11785 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 10
Calculated molecular weight 464 aa, 53 kDa
GenBank accession numberBC065516
Gene Symbol JNK3
Gene ID (NCBI) 5602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP53779
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.