Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, HeLa cells, MDA-MB-468 cells, SH-SY5Y cells, SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27573-1-AP targets MAP7D3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26082 Product name: Recombinant human MAP7D3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 301-400 aa of NM_001173516 Sequence: ASVDAPPQVNVEVFCNTSMEASPKAGVGMAPEVSTDSFPVVSVDVSPVVSTYDSEMSMDASPELSIEALPKVDLETVPKVSIVASPEASLEAPPEVSLEA 相同性解析による交差性が予測される生物種 |
| Full Name | MAP7 domain containing 3 |
| Calculated molecular weight | 98 kDa |
| Observed molecular weight | 125-130 kDa |
| GenBank accession number | NM_001173516 |
| Gene Symbol | MAP7D3 |
| Gene ID (NCBI) | 79649 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWC1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP7D3, also known as MDP3, features two coiled-coil regions near its N terminus, followed by a linker region, a MAP7-like domain, and a C-terminal domain. MAP7D3 is a microtubule-binding protein that regulates microtubule assembly and stability. MAP7D3 expression is cell cycle dependent, with lower expression in the G2 phase compared to its expression in the other phases of the cell cycle(PMID: 22142902).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP7D3 antibody 27573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |