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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of A549 using 13446-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of HeLa using 13446-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of A431 using 13446-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IP experiment of HeLa using 13446-1-AP

    IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using MAP7 Polyclonal antibody (13446-1-AP)

    IHC staining of human colon using 13446-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HeLa using 13446-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP7 antibody (13446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HeLa using 13446-1-AP

    Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HepG2 using 13446-1-AP

    Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MAP7 Polyclonal antibody

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13446-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13446-1-AP targets MAP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4276

Product name: Recombinant human MAP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-749 aa of BC025777

Sequence: GRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREWEEAERAQRQKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 7
Calculated molecular weight 749 aa, 84 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025777
Gene Symbol MAP7
Gene ID (NCBI) 9053
RRIDAB_10644485
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAP7 antibody 13446-1-APDownload protocol
IHC protocol for MAP7 antibody 13446-1-APDownload protocol
IF protocol for MAP7 antibody 13446-1-APDownload protocol
IP protocol for MAP7 antibody 13446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

BMC Biol

Two-step interphase microtubule disassembly aids spindle morphogenesis.

Authors - Nunu Mchedlishvili
View Article
humanIF

Mol Biol Cell

Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.

Authors - Erin M Masucci
View Article
humanWB,IHC

Adv Sci (Weinh)

N6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation

Authors - Yin Zhao
View Article
humanWB

Front Pharmacol

A telomere-related signature for predicting prognosis and assessing immune microenvironment in osteosarcoma

Authors - Shihao Li
View Article