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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of mouse brain using 55244-1-AP (same clone as 55244-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of human brain using 55244-1-AP (same clone as 55244-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MAP4K2 Polyclonal antibody (55244-1-AP)

    WB analysis of Raji using 55244-1-AP (same clone as 55244-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4K2 Polyclonal antibody (55244-1-AP)

    IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

MAP4K2 Polyclonal antibody, PBS Only

MAP4K2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55244-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

B lymphocyte serine/threonine-protein kinase, EC:2.7.11.1, GCK, MEKKK 2, Mitogen-activated protein kinase kinase kinase kinase 2

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55244-1-PBS targets MAP4K2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase kinase 2
Calculated molecular weight 92 kDa
Observed molecular weight 85-91 kDa, 58 kDa
GenBank accession numberNM_004579
Gene Symbol MAP4K2
Gene ID (NCBI) 5871
RRIDAB_10860908
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12851
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.

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