Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Jurkat cells, Raji cells, Ramos cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
23950-1-AP targets MAP4K1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20221 Product name: Recombinant human MAP4K1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 364-551 aa of BC152994 Sequence: LRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNV 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase kinase kinase 1 |
| Calculated molecular weight | 833 aa, 91 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC152994 |
| Gene Symbol | MAP4K1 |
| Gene ID (NCBI) | 11184 |
| RRID | AB_2879372 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q92918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP4K1 antibody 23950-1-AP | Download protocol |
| IP protocol for MAP4K1 antibody 23950-1-AP | Download protocol |
| WB protocol for MAP4K1 antibody 23950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells. | ||
Clin Exp Med An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma | ||
Nat Commun DNA framework-engineered chimeras platform enables selectively targeted protein degradation | ||
Oncogene CBX2 as a therapeutic target in colorectal cancer: insights into the altered chromatin accessibility via RUNX1-CBX2-MAP4K1 axis |
