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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP3K6 Polyclonal antibody

MAP3K6 Polyclonal Antibody for IP,IHC,IF,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 16212-1-AP

Synonyms

ASK2, MAP3K6, MAPKKK6, MEKK6

Validation Data Gallery

Applications:
  • IP experiment of mouse liver using 16212-1-AP

    IP experiment of mouse liver using 16212-1-AP

    IP result of anti-MAP3K6 (IP:16212-1-AP, 4ug; Detection:16212-1-AP 1:300) with mouse liver tissue lysate 4000ug.

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 16212-1-AP

    IHC staining of human liver cancer using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16212-1-AP

    IHC staining of human liver cancer using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 16212-1-AP

    IHC staining of human liver cancer using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16212-1-AP

    IHC staining of human liver cancer using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 16212-1-AP

    IF Staining of HeLa using 16212-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAP3K6 antibody (16212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

16212-1-AP targets MAP3K6 in IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP3K6 fusion protein Ag9206 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 6
Calculated molecular weight1148aa,128 kDa; 1011aa,113 kDa; 797aa,89 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC015914
Gene symbol MAP3K6
Gene ID (NCBI) 9064
RRIDAB_2878229
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MAP3K6 antibody 16212-1-APDownload protocol
IF protocol for MAP3K6 antibody 16212-1-APDownload protocol
IP protocol for MAP3K6 antibody 16212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols