Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
11996-1-AP targets MLK3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2636 Product name: Recombinant human MAP3K11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-379 aa of BC011263 Sequence: MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEAL 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase kinase 11 |
| Calculated molecular weight | 847 aa, 93 kDa |
| Observed molecular weight | 93 and 65 kDa |
| GenBank accession number | BC011263 |
| Gene Symbol | MLK3 |
| Gene ID (NCBI) | 4296 |
| RRID | AB_10694137 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16584 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MLK3 antibody 11996-1-AP | Download protocol |
| IHC protocol for MLK3 antibody 11996-1-AP | Download protocol |
| WB protocol for MLK3 antibody 11996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Metabolism Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier. | ||
Cell Death Dis Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload. | ||
Acta Pharmacol Sin Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy. | ||
Int J Mol Med Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway. | ||
J Ethnopharmacol Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload. | ||
Biochem Pharmacol 2'-O-Galloylhyperin attenuates osteoclastogenesis and estrogen-deficiency osteoporosis by targeting MLK3-mediated NF-κB signaling. |