• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP3K10 Polyclonal antibody

MAP3K10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29985-1-AP

Synonyms

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

Validation Data Gallery

Applications:
  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Sample-dependent, check data in validation data gallery

Product Information

29985-1-AP targets MAP3K10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP3K10 fusion protein Ag32419 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 10
Calculated molecular weight104KD
Observed molecular weight104-115 kDa
GenBank accession numberNM_002446
Gene symbol MAP3K10
Gene ID (NCBI) 4294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. MAP3K10 can be detected 104-115 kDa by phosphorylation (PMID: 23178452).

Protocols

Product Specific Protocols
WB protocol for MAP3K10 antibody 29985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols