Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29985-1-AP targets MLK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32419 Product name: Recombinant human MAP3K10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 476-620 aa of NM_002446 Sequence: LPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAED 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase kinase 10 |
| Calculated molecular weight | 104KD |
| Observed molecular weight | 104-115 kDa |
| GenBank accession number | NM_002446 |
| Gene Symbol | MAP3K10 |
| Gene ID (NCBI) | 4294 |
| RRID | AB_3086204 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02779 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. MAP3K10 can be detected 104-115 kDa by phosphorylation (PMID: 23178452).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MLK2 antibody 29985-1-AP | Download protocol |
| WB protocol for MLK2 antibody 29985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |