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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51142-1-AP

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 51142-1-AP

    WB analysis of mouse brain using 51142-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 51142-1-AP

    WB analysis of HeLa using 51142-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 51142-1-AP

    WB analysis of mouse brain using 51142-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 51142-1-AP

    IHC staining of human breast cancer using 51142-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 51142-1-AP

    IHC staining of human breast cancer using 51142-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51142-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2881252
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAP2K4 antibody 51142-1-APDownload protocol
IHC protocol for MAP2K4 antibody 51142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols