Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 2 publications below |
Product Information
17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11376 Product name: Recombinant human MAP2K4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-399 aa of BC036032 Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase 4 |
| Calculated molecular weight | 399 aa, 44 kDa |
| Observed molecular weight | 44-50 kDa |
| GenBank accession number | BC036032 |
| Gene Symbol | MAP2K4 |
| Gene ID (NCBI) | 6416 |
| RRID | AB_2878389 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| WB protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. | ||
Int J Biochem Cell Biol Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.
| ||
Dig Dis Sci LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4. |
