Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 14600-1-AP
WB result of LC3 antibody (14600-1-AP; 1:6000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells.
WB analysis using 14600-1-AP
Non-treated and Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 14600-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14600-1-AP
IP result of anti-LC3 (IP:14600-1-AP, 4ug; Detection:14600-1-AP 1:3000) with HeLa cells lysate 1600 ug.
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14600-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14600-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 14600-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human LC3 (14600-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14600-1-AP

Publications(2431)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2431)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, Chloroquine treated HeLa cells, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue, human gliomas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 1969 publications below
IHC	See 226 publications below
IF	See 652 publications below
IP	See 10 publications below
ELISA	See 1 publications below
CoIP	See 10 publications below

Product Information

14600-1-AP targets LC3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, monkey, chicken, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6144 Product name: Recombinant human LC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC067797 Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV 相同性解析による交差性が予測される生物種
Full Name	microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight	15 kDa
Observed molecular weight	14-18 kDa
GenBank accession number	BC067797
Gene Symbol	LC3B
Gene ID (NCBI)	81631
ENSEMBL Gene ID	ENSG00000140941
RRID	AB_2137737
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZQ8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
FC protocol for LC3 antibody 14600-1-AP	Download protocol
IF protocol for LC3 antibody 14600-1-AP	Download protocol
IHC protocol for LC3 antibody 14600-1-AP	Download protocol
IP protocol for LC3 antibody 14600-1-AP	Download protocol
WB protocol for LC3 antibody 14600-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. Authors - Wenchang Xiao View Article
human	WB	Signal Transduct Target Ther Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation Authors - Guan-Nan Li View Article
human	WB,IF	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
mouse	IF	Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease Authors - Boyuan Ma View Article
human	WB	Nature PtdIns(3,5)P(2) is an endogenous ligand of STING in innate immune signalling. Authors - Jay Xiaojun Tan View Article
mouse	WB	Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling Authors - Kaixin Ding View Article

Validation Data Gallery

WB analysis of HeLa using 14600-1-AP

WB analysis using 14600-1-AP

WB analysis using 14600-1-AP

IP experiment of HeLa using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of human gliomas using 14600-1-AP

IHC staining of human liver using 14600-1-AP

IHC staining of human liver using 14600-1-AP

IHC staining of human gliomas using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IHC staining of mouse brain using 14600-1-AP

IF Staining of HeLa using 14600-1-AP

IF Staining of HepG2 using 14600-1-AP

IF Staining of HepG2 using 14600-1-AP

FC experiment of HeLa using 14600-1-AP

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

PtdIns(3,5)P(2) is an endogenous ligand of STING in innate immune signalling.

Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling

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