Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87623-1-RR targets MAP1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein 1A |
| Calculated molecular weight | 305 kDa |
| Observed molecular weight | 26-28 kDa |
| GenBank accession number | NM_002373 |
| Gene Symbol | MAP1A |
| Gene ID (NCBI) | 4130 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The assembly of microtubules, an essential step in neurogenesis, is modulated by the family of microtubule-associated proteins (MAPs). These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B, and MAP2, and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1A is the largest brain MAP and is expressed prominently in dendrites and to a lesser extent in cell bodies and axons. This protein is involved in differentiation of axons and dendrites as well as maintenance of the microtubule cytoskeleton in mature neurons. This antibody recognizes MAP1 light chain LC2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP1A antibody 87623-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |