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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human kidney using 66107-1-Ig (same clone as 66107-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IF Staining of mouse kidney using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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MAOB Monoclonal antibody, PBS Only

MAOB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66107-1-PBS
Clone No.2B12F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66107-1-PBS targets MAOB in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAOB fusion protein Ag17918 相同性解析による交差性が予測される生物種
Full Name monoamine oxidase B
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC022494
Gene Symbol MAOB
Gene ID (NCBI) 4129
RRIDAB_2881506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27338
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).