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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis of LNCaP using 10539-1-AP (same clone as 10539-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis using 10539-1-AP (same clone as 10539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis of human placenta using 10539-1-AP (same clone as 10539-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of LNCaP cells using MAOA Polyclonal antibody (10539-1-AP)

    IP experiment of LNCaP using 10539-1-AP (same clone as 10539-1-PBS)

    IP result of anti-MAOA (IP:10539-1-AP, 4ug; Detection:10539-1-AP 1:5000) with LNCaP cells lysate 880 ug. This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP (same clone as 10539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP (same clone as 10539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP (same clone as 10539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP (same clone as 10539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP (same clone as 10539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10539-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MAOA Polyclonal antibody, PBS Only

MAOA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Amine oxidase [flavin-containing] A, EC:1.4.3.21, MAO A, MAO-A, monoamine oxidase A

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10539-1-PBS targets MAOA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0821

Product name: Recombinant human MAOA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-527 aa of BC008064

Sequence: HPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS

相同性解析による交差性が予測される生物種
Full Name monoamine oxidase A
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC008064
Gene Symbol MAOA
Gene ID (NCBI) 4128
RRIDAB_2137251
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP21397
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

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