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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MANEA Polyclonal antibody

MANEA Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21296-1-AP

Synonyms

ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 21296-1-AP

    WB analysis of HEK-293 using 21296-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 21296-1-AP

    WB analysis of mouse kidney using 21296-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:100 incubated at 4 degree celsius over night.

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IF Staining of HEK-293 using 21296-1-AP

    IF Staining of HEK-293 using 21296-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21296-1-AP (MANEA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21296-1-AP is MANEA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MANEA fusion protein Ag15847 相同性解析による交差性が予測される生物種
Full Name mannosidase, endo-alpha
Calculated molecular weight 462 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC146671
Gene symbol MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MANEA antibody 21296-1-APDownload protocol
IHC protocol for MANEA antibody 21296-1-APDownload protocol
IF protocol for MANEA antibody 21296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols