Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19189-1-AP targets MAN2C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6284 Product name: Recombinant human MAN2C1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 691-1040 aa of BC050550 Sequence: NGIIRVKLDPTGRLTSLVLVASGREAIAEGAVGNQFVLFDDVPLYWDAWDVMDYHLETRKPVLGQAGTLAVGTEGGLRGSAWFLLQISPNSRLSQEVVLDVGCPYVRFHTEVHWHEAHKFLKVEFPARVRSSQATYEIQFGHLQRPTHYNTSWDWARFEVWAHRWMDLSEHGFGLALLNDCKYGASVRGSILSLSLLRAPKAPDATADTGRHEFTYALMPHKGSFQDAGVIQAAYSLNFPLLALPAPSPAPATSWSAFSVSSPAVVLETVKQAESSPQRRSLVLRLYEAHGSHVDCWLHLSLPVQEAILCDLLERPDPAGHLTLRDNRLKLTFSPFQVLSLLLVLQPPPH 相同性解析による交差性が予測される生物種 |
| Full Name | mannosidase, alpha, class 2C, member 1 |
| Calculated molecular weight | 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC050550 |
| Gene Symbol | MAN2C1 |
| Gene ID (NCBI) | 4123 |
| RRID | AB_3669307 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NTJ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha mannosidase 2C1(MAN2C1) an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol. MAN2C1 cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmatic free oligosaccharides generated by N-glycoprotein degradation pathways (PMID: 16848760). Impaired catabolism of free oligosaccharides due to MAN2C1 variants causes a neurodevelopmental disorder (PMID: 35045343).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAN2C1 antibody 19189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |