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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN1A2 Polyclonal antibody

MAN1A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14049-1-AP

Synonyms

Alpha 1,2 mannosidase IB, MAN1A2, MAN1B

Validation Data Gallery

Applications:
  • WB analysis of human placenta using 14049-1-AP

    WB analysis of human placenta using 14049-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 14049-1-AP

    IP experiment of human placenta using 14049-1-AP

    IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug.

  • IHC staining of human placenta using 14049-1-AP

    IHC staining of human placenta using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 14049-1-AP

    IHC staining of human placenta using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 14049-1-AP

    IHC staining of human lung using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 14049-1-AP

    IHC staining of human lung using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary using 14049-1-AP

    IHC staining of human ovary using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 14049-1-AP

    IHC staining of human ovary using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 14049-1-AP

    IHC staining of human heart using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 14049-1-AP

    IHC staining of human heart using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human heart tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14049-1-AP targets MAN1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAN1A2 fusion protein Ag5240 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1A, member 2
Calculated molecular weight 641 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC063300
Gene symbol MAN1A2
Gene ID (NCBI) 10905
RRIDAB_2266033
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).

Protocols

Product Specific Protocols
WB protocol for MAN1A2 antibody 14049-1-APDownload protocol
IHC protocol for MAN1A2 antibody 14049-1-APDownload protocol
IP protocol for MAN1A2 antibody 14049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Gastroenterol

Hepatitis B virus upregulates host expression of α-1,2-mannosidases via the PPARα pathway.

Authors - Song Hu
View Article