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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAN1A1 Recombinant antibody (86094-1-RR)

    WB analysis using 86094-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86094-1-RR (MAN1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MAN1A1 Recombinant antibody (86094-1-RR)

    IHC staining of human ovary cancer using 86094-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86094-1-RR (MAN1A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAN1A1 Recombinant antibody (86094-1-RR)

    IF Staining of HepG2 using 86094-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (86094-1-RR, Clone: 250444H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86094-1-RR

    Affinity and Kinetic Characterization of 86094-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86094-1-RR against Human MAN1A1 were performed. The affinity constant is 1.04 nM.

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MAN1A1 Recombinant antibody

MAN1A1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86094-1-RR
Clone No.250444H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HEK-293 cells, human urine
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86094-1-RR targets MAN1A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAN1A1 fusion protein Ag20060 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1A, member 1
Calculated molecular weight 653 aa, 73 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC065827
Gene Symbol MAN1A1
Gene ID (NCBI) 4121
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

Protocols

Product Specific Protocols
WB protocol for MAN1A1 antibody 86094-1-RRDownload protocol
IHC protocol for MAN1A1 antibody 86094-1-RRDownload protocol
IF protocol for MAN1A1 antibody 86094-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols