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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAK16 Polyclonal antibody (17505-1-AP)

    WB analysis using 17505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17505-1-AP (MAK16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAK16 Polyclonal antibody (17505-1-AP)

    IF Staining of A431 using 17505-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAK16 antibody (17505-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MAK16 Polyclonal antibody

MAK16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17505-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RBM13, Protein RBM13, Protein MAK16 homolog, NNP78

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17505-1-AP targets MAK16 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAK16 fusion protein Ag11705 相同性解析による交差性が予測される生物種
Full Name MAK16 homolog (S. cerevisiae)
Calculated molecular weight 300 aa, 35 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC050528
Gene Symbol MAK16
Gene ID (NCBI) 84549
RRIDAB_2281504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAK16 antibody 17505-1-APDownload protocol
IF protocol for MAK16 antibody 17505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols