Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 17430-1-AP (same clone as 17430-1-PBS)
Mouse cerebellum lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
WB analysis using 17430-1-AP (same clone as 17430-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 17430-1-AP (same clone as 17430-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 17430-1-AP (same clone as 17430-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 17430-1-AP (same clone as 17430-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 17430-1-AP (same clone as 17430-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 17430-1-AP (same clone as 17430-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17430-1-AP (MAGT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGT1 antibody (17430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGT1 antibody (17430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 17430-1-AP (same clone as 17430-1-PBS)
Immunofluorescent analysis of HepG2 cells, using 17430-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.
IF Staining of C2C12 using 17430-1-AP (same clone as 17430-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MAGT1 antibody (17430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17430-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MAGT1 Polyclonal antibody, PBS Only

MAGT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 17430-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

bA217H1.1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1, IAG2, IAP, Implantation-associated protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17430-1-PBS targets MAGT1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11496 Product name: Recombinant human MAGT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-182 aa of BC060842 Sequence: KKEMVLSEKVSQLMEWTNKRPVIRMNGDKFRRLVKAPPRNYSVIVMFTALQLHRQCVVCKQADEEFQILANSWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFPAKGKPKRGDTYELQVRGFSAEQIARWIADRTDVNIRVIR 相同性解析による交差性が予測される生物種
Full Name	magnesium transporter 1
Calculated molecular weight	335 aa, 38 kDa
Observed molecular weight	45-47 kDa
GenBank accession number	BC060842
Gene Symbol	MAGT1
Gene ID (NCBI)	84061
RRID	AB_2138982
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0U3
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17430-1-AP (same clone as 17430-1-PBS)

WB analysis using 17430-1-AP (same clone as 17430-1-PBS)

WB analysis of mouse heart using 17430-1-AP (same clone as 17430-1-PBS)

WB analysis of mouse colon using 17430-1-AP (same clone as 17430-1-PBS)

WB analysis of HEK-293 using 17430-1-AP (same clone as 17430-1-PBS)

WB analysis of COLO 320 using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of human liver using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of human liver using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of human skeletal muscle using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of human skeletal muscle using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)

IHC staining of human colon cancer using 17430-1-AP (same clone as 17430-1-PBS)

IF Staining of mouse brain using 17430-1-AP (same clone as 17430-1-PBS)

IF Staining of HepG2 using 17430-1-AP (same clone as 17430-1-PBS)

IF Staining of C2C12 using 17430-1-AP (same clone as 17430-1-PBS)