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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGI3 Recombinant antibody (84771-4-RR)

    WB analysis using 84771-4-RR (same clone as 84771-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84771-4-RR (MAGI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAGI3 Recombinant antibody (84771-4-RR)

    IHC staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84771-4-RR (MAGI3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAGI3 Recombinant antibody (84771-4-RR)

    IF Staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGI3 antibody (84771-4-RR, Clone: 242212C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84771-4-RR

    Affinity and Kinetic Characterization of 84771-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84771-4-RR against Human MAGI3 were performed. The affinity constant is 0.752 nM.

MAGI3 Recombinant monoclonal antibody, PBS Only

MAGI3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84771-4-PBS
Clone No.242212C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242212C9, KIAA1634, MAGI-3, Membrane-associated guanylate kinase inverted 3, Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84771-4-PBS targets MAGI3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18394

Product name: Recombinant human MAGI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-777 aa of BC130409

Sequence: LPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCID

相同性解析による交差性が予測される生物種
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 3
Calculated molecular weight 1481 aa, 163 kDa
Observed molecular weight140 kDa
GenBank accession numberBC130409
Gene Symbol MAGI3
Gene ID (NCBI) 260425
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ5TCQ9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

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