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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MAGED4 Polyclonal antibody (16809-1-AP)

    WB analysis of Jurkat using 16809-1-AP (same clone as 16809-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MAGED4 Polyclonal antibody (16809-1-AP)

    WB analysis of A549 using 16809-1-AP (same clone as 16809-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MAGED4 Polyclonal antibody (16809-1-AP)

    IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MAGED4 Polyclonal antibody (16809-1-AP)

    IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MAGED4 Polyclonal antibody (16809-1-AP)

    IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MAGED4 Polyclonal antibody (16809-1-AP)

    IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

MAGED4 Polyclonal antibody, PBS Only

MAGED4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16809-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16809-1-PBS targets MAGED4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10329

Product name: Recombinant human MAGED4 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-350 aa of BC081566

Sequence: MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSK

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family D, 4
Calculated molecular weight 741 aa, 81 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC081566
Gene Symbol MAGED4
Gene ID (NCBI) 728239
RRIDAB_2138678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JG8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).

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