Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15252-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15252-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15252-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15252-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15252-1-AP
IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug.
IHC staining of human breast cancer using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 15252-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

Featured Product
KD/KO Validated

MAGED2 Polyclonal antibody

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15252-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

11B6, BCG-1, Breast cancer-associated gene 1 protein, HCA10, Hepatocellular carcinoma-associated protein JCL-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue, mouse small intestine tissue, mouse pancreas tissue, rat kidney tissue, mouse kidney tissue, rat pancreas tissue, human placenta tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6828 Product name: Recombinant human MAGED2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-479 aa of BC000304 Sequence: DEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAM 相同性解析による交差性が予測される生物種
Full Name	melanoma antigen family D, 2
Calculated molecular weight	65 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC000304
Gene Symbol	MAGED2
Gene ID (NCBI)	10916
RRID	AB_2138557
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNF1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for MAGED2 antibody 15252-1-AP	Download protocol
IHC protocol for MAGED2 antibody 15252-1-AP	Download protocol
IP protocol for MAGED2 antibody 15252-1-AP	Download protocol
WB protocol for MAGED2 antibody 15252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Nephrol Dial Transplant MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells. Authors - Lara Valiño-Rivas View Article
human	WB	Biochem Pharmacol Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response. Authors - Charlotte Trussart KD Validated View Article
human	WB,IP	mBio SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense Authors - Xiaohui Ju KD Validated View Article
human	WB	RNA An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21. Authors - Ewan K S McRae View Article
mouse	WB	J Neurosci MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. Authors - Mouri Akihiro A View Article
human	WB,IF	Biochim Biophys Acta Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress. Authors - Céline Pirlot KD Validated View Article

Validation Data Gallery

WB analysis using 15252-1-AP

WB analysis of HepG2 using 15252-1-AP

WB analysis of A431 using 15252-1-AP

WB analysis of MCF-7 using 15252-1-AP

IP experiment of HepG2 using 15252-1-AP

IHC staining of human breast cancer using 15252-1-AP

IHC staining of human breast cancer using 15252-1-AP

IHC staining of human placenta using 15252-1-AP

IHC staining of mouse pancreas using 15252-1-AP

IHC staining of mouse small intestine using 15252-1-AP

IHC staining of rat kidney using 15252-1-AP

IHC staining of rat pancreas using 15252-1-AP

IHC staining of rat small intestine using 15252-1-AP

IHC staining of mouse kidney using 15252-1-AP

IF Staining of MCF-7 using 15252-1-AP

MAGED2 Polyclonal antibody

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells.

Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.

SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense

An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.

MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.

Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.

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