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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    WB analysis of HepG2 using 12400-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    WB analysis of A375 using 12400-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IP experiment of HepG2 using 12400-1-AP

    IP result of anti-MAGEB1 (IP:12400-1-AP, 4ug; Detection:12400-1-AP 1:2000) with HepG2 cells lysate 3360 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IF Staining of HepG2 using 12400-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB1 antibody (12400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IF Staining of A375 using 12400-1-AP

    Immunofluorescent analysis of A375 cells using 12400-1-AP (MAGEB1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12400-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A375 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12400-1-AP targets MAGEB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3094

Product name: Recombinant human MAGEB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC013772

Sequence: MPRGQKSKLRAREKRRKAREETHYLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family B, 1
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC013772
Gene Symbol MAGEB1
Gene ID (NCBI) 4112
RRIDAB_2877854
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43366
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.

Protocols

Product Specific Protocols
IF protocol for MAGEB1 antibody 12400-1-APDownload protocol
IP protocol for MAGEB1 antibody 12400-1-APDownload protocol
WB protocol for MAGEB1 antibody 12400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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