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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA8 Polyclonal antibody

MAGEA8 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15045-1-AP

Synonyms

Cancer/testis antigen 1.8, CT1.8, MAGE 8 antigen, MAGE8, MAGEA8, melanoma antigen family A, 8, Melanoma associated antigen 8

Validation Data Gallery

Applications:
  • WB analysis of A375 using 15045-1-AP

    WB analysis of A375 using 15045-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15045-1-AP (MAGEA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15045-1-AP

    IHC staining of human testis using 15045-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 15045-1-AP

    IHC staining of human testis using 15045-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A375 using 15045-1-AP

    IF Staining of A375 using 15045-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 15045-1-AP (MAGEA8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15045-1-AP targets MAGEA8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA8 fusion protein Ag6984 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 8
Calculated molecular weight 35 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC002455
Gene symbol MAGEA8
Gene ID (NCBI) 4107
RRIDAB_2138289
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA8 antibody 15045-1-APDownload protocol
IHC protocol for MAGEA8 antibody 15045-1-APDownload protocol
IF protocol for MAGEA8 antibody 15045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols