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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA6 Polyclonal antibody (14602-1-AP)

    WB analysis using 14602-1-AP (same clone as 14602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAGEA6 Polyclonal antibody (14602-1-AP)

    WB analysis using 14602-1-AP (same clone as 14602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human lung cancer using 14602-1-AP (same clone as 14602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human lung cancer using 14602-1-AP (same clone as 14602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14602-1-AP (MAGEA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human breast cancer using 14602-1-AP (same clone as 14602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEA6 Polyclonal antibody (14602-1-AP)

    IHC staining of human breast cancer using 14602-1-AP (same clone as 14602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA6 Polyclonal antibody (14602-1-AP)

    IF Staining of A375 using 14602-1-AP (same clone as 14602-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using MAGEA6 antibody (14602-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14602-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MAGEA6 Polyclonal antibody, PBS Only

MAGEA6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14602-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14602-1-PBS targets MAGEA6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6148

Product name: Recombinant human MAGEA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC067731

Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAKLVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFIKASDSLQLVFGIELMEVDPIGHVYIFATCLGLSYDGLLGDNQIMPKTGFLIIILAIIAKEGDCAPEEKIWEELSVLEVFEGREDSIFGDPKKLLTQYFVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHMVKISGGPRISYPLLHEWALREGEE

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 6
Calculated molecular weight 35 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC067731
Gene Symbol MAGEA6
Gene ID (NCBI) 4105
RRIDAB_2250093
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43360
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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