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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis using 84248-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis of human testis using 84248-6-RR

    human testis tissue was subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAGEA4 Recombinant antibody (84248-6-RR)

    IF Staining of A431 using 84248-6-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAGEA4 antibody (84248-6-RR, Clone: 241558B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of A431 using 84248-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of U-2 OS using 84248-6-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

MAGEA4 Recombinant monoclonal antibody

MAGEA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84248-6-RR
Clone No.241558B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241558B10, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, human testis tissue, U2OS cells, A375 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84248-6-RR targets MAGEA4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3193

Product name: Recombinant human MAGEA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC017723

Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_3671798
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP43358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.

Protocols

Product Specific Protocols
IF protocol for MAGEA4 antibody 84248-6-RRDownload protocol
WB protocol for MAGEA4 antibody 84248-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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