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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Recombinant antibody (84248-4-RR)

    WB analysis using 84248-4-RR (same clone as 84248-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using MAGEA4 Recombinant antibody (84248-4-RR)

    WB analysis of human testis using 84248-4-RR (same clone as 84248-4-PBS)

    human testis tissue was subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using MAGEA4 Recombinant antibody (84248-4-RR)

    FC experiment of A431 using 84248-4-RR (same clone as 84248-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using MAGEA4 Recombinant antibody (84248-4-RR)

    FC experiment of U2OS using 84248-4-RR (same clone as 84248-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84248-4-RR

    Affinity and Kinetic Characterization of 84248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

MAGEA4 Recombinant monoclonal antibody, PBS Only

MAGEA4 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84248-4-PBS
Clone No.241558G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

241558G7, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84248-4-PBS targets MAGEA4 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3193

Product name: Recombinant human MAGEA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC017723

Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight45 kDa
GenBank accession numberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43358
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

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