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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA3 Recombinant antibody (83480-5-RR)

    WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAGEA3 Recombinant antibody (83480-5-RR)

    WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MAGEA3 Recombinant antibody (83480-5-RR)

    IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MAGEA3 Recombinant antibody (83480-5-RR)

    FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using MAGEA3 Recombinant antibody (83480-5-RR)

    FC experiment of Jurkat using 83480-5-RR (same clone as 83480-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83480-5-RR

    Affinity and Kinetic Characterization of 83480-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

MAGEA3 Recombinant monoclonal antibody, PBS Only

MAGEA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83480-5-PBS
Clone No.240435G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240435G9, Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83480-5-PBS targets MAGEA3 in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0265

Product name: Recombinant human MAGEA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of BC000340

Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLE

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 3
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight45 kDa
GenBank accession numberBC000340
Gene Symbol MAGEA3
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43357
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.