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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA10 Polyclonal antibody (15295-1-AP)

    WB analysis of A375 using 15295-1-AP (same clone as 15295-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MAGEA10 Polyclonal antibody (15295-1-AP)

    WB analysis of Jurkat using 15295-1-AP (same clone as 15295-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15295-1-PBS in a different storage buffer formulation.

MAGEA10 Polyclonal antibody, PBS Only

MAGEA10 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 15295-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15295-1-PBS targets MAGEA10 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7540

Product name: Recombinant human MAGEA10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-369 aa of BC004105

Sequence: SSSTSTSSSFPSSFPSSSSSSSSSCYPLIPSTPEEVSADDETPNPPQSAQIACSSPSVVASLPLDQSDEGSSSQKEESPSTLQVLPDSESLPRSEIDEKVTDLVQFLLFKYQMKEPITKAEILESVIKNYEDHFPLLFSEASECMLLVFGIDVKEVDPTGHSFVLVTSLGLTYDGMLSDVQSMPKTGILILILSIIFIEGYCTPEEVIWEALNMMGLYDGMEHLIYGEPRKLLTQDWVQENYLEYRQVPGSDPARYEFLWGPRAHAEIRKMSLLKFLAKVNGSDPRSFPLWYEEALKDEEERAQDRIATTDDTTAMASASSSATGSFSYPE

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 10
Calculated molecular weight 41 kDa
Observed molecular weight 62 kDa, 41 kDa
GenBank accession numberBC004105
Gene Symbol MAGEA10
Gene ID (NCBI) 4109
RRIDAB_2137968
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43363
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.