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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA1 Polyclonal antibody (12233-1-AP)

    WB analysis of A375 using 12233-1-AP (same clone as 12233-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12233-1-AP (MAGEA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12233-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA1 Polyclonal antibody (12233-1-AP)

    IHC staining of human testis using 12233-1-AP (same clone as 12233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12233-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA1 Polyclonal antibody (12233-1-AP)

    IHC staining of human testis using 12233-1-AP (same clone as 12233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12233-1-PBS in a different storage buffer formulation.

MAGEA1 Polyclonal antibody, PBS Only

MAGEA1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12233-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12233-1-PBS targets MAGEA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2871

Product name: Recombinant human MAGEA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-309 aa of BC017555

Sequence: MSLEQRSLHCKPEEALEAQQEALGLVCVQAAASSSSPLVLGTLEEVPTAGSTDPPQSPQGASAFPTTINFTRQRQPSEGSSSREEEGPSTSCILESLFRAVITKKVADLVGFLLLKYRAREPVTKAEMLESVIKNYKHCFPEIFGKASESLQLVFGIDVKEADPTGHSYVLVTCLGLSYDGLLGDNQIMPKTGFLIIVLVMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV

相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
Calculated molecular weight 309 aa, 34 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC017555
Gene Symbol MAGEA1
Gene ID (NCBI) 4100
RRIDAB_2250080
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43355
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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