Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, J774A.1 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11619-1-AP targets MAF1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2198 Product name: Recombinant human MAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC018714 Sequence: MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGAEETSTMEEDRVPVICI 相同性解析による交差性が予測される生物種 |
| Full Name | MAF1 homolog (S. cerevisiae) |
| Calculated molecular weight | 256 aa, 29 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC018714 |
| Gene Symbol | MAF1 |
| Gene ID (NCBI) | 84232 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H063 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAF1 operates as a conserved repressor of RNA polymerase III transcription, first identified in yeast. It binds RNA pol III directly, blocking TFIIIB recruitment and dampening tRNA and 5S rRNA output. In mammals, MAF1 additionally fine-tunes select RNA pol II genes, impacting metabolism, bone formation, adipogenesis, and oncogenesis, while its own activity is stringently controlled by mTORC1 phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAF1 antibody 11619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |