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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-MAF Polyclonal antibody (55013-1-AP)

    WB analysis using 55013-1-AP (same clone as 55013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55013-1-AP (c-MAF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using c-MAF Polyclonal antibody (55013-1-AP)

    WB analysis of HepG2 using 55013-1-AP (same clone as 55013-1-PBS)

    WB result of c-MAF antibody (55013-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HepG2 cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using c-MAF Polyclonal antibody (55013-1-AP)

    WB analysis of HeLa using 55013-1-AP (same clone as 55013-1-PBS)

    WB result of c-MAF antibody (55013-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HeLa cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using c-MAF Polyclonal antibody (55013-1-AP)

    WB analysis of A375 using 55013-1-AP (same clone as 55013-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 55013-1-AP (c-MAF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using c-MAF Polyclonal antibody (55013-1-AP)

    IP experiment of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    IP result of anti-c-MAF (IP:55013-1-AP, 4ug; Detection:55013-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using c-MAF Polyclonal antibody (55013-1-AP)

    IF Staining of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 55013-1-AP (c-MAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

c-MAF Polyclonal antibody, PBS Only

c-MAF Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 55013-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

MAF, c MAF, cMAF, Proto oncogene c Maf, Proto-oncogene c-Maf

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55013-1-PBS targets c-MAF in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
Calculated molecular weight 42 kDa
Observed molecular weight 42-52 kDa
GenBank accession numberNM_005360
Gene Symbol MAF
Gene ID (NCBI) 4094
RRIDAB_10863127
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75444
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .

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