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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

    WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of Jurkat using 66014-1-Ig (same clone as 66014-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of COLO 320 using 66014-1-Ig (same clone as 66014-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAD2L1 Monoclonal antibody (66014-1-Ig)

    WB analysis of HeLa using 66014-1-Ig (same clone as 66014-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAD2L1 Monoclonal antibody (66014-1-Ig)

    IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAD2L1 Monoclonal antibody (66014-1-Ig)

    IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MAD2L1 Monoclonal antibody, PBS Only

MAD2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66014-1-PBS
Clone No.2F5F12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

2F5F12, HSMAD2, MAD2, MAD2 like protein 1, MAD2-like protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66014-1-PBS targets MAD2L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17512

Product name: Recombinant human MAD2L1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 16-185 aa of BC000356

Sequence: SAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRS

相同性解析による交差性が予測される生物種
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC000356
Gene Symbol MAD2L1
Gene ID (NCBI) 4085
RRIDAB_11232424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13257
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

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