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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using M-cadherin Recombinant antibody (84440-5-RR)

    WB analysis of mouse cerebellum using 84440-5-RR

    mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 84440-5-RR (CDH15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using M-cadherin Recombinant antibody (84440-5-RR)

    IHC staining of mouse skeletal muscle using 84440-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84440-5-RR (M-cadherin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using M-cadherin Recombinant antibody (84440-5-RR)

    IF Staining of mouse skeletal muscle using 84440-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using M-cadherin antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using M-cadherin Recombinant antibody (84440-5-RR)

    IF Staining of C2C12 using 84440-5-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CDH15 antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84440-5-RR

    Affinity and Kinetic Characterization of 84440-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84440-5-RR against Human M-cadherin were performed. The affinity constant is below 1 pM.

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M-cadherin Recombinant antibody

M-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 84440-5-RR
Clone No.241837C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84440-5-RR targets M-cadherin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen M-cadherin fusion protein Ag25763 相同性解析による交差性が予測される生物種
Full Name cadherin 15, type 1, M-cadherin (myotubule)
Calculated molecular weight 89 kDa
Observed molecular weight90-130 kDa
GenBank accession numberBC008951
Gene Symbol M-cadherin
Gene ID (NCBI) 1013
RRIDAB_3671966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).

Protocols

Product Specific Protocols
WB protocol for M-cadherin antibody 84440-5-RRDownload protocol
IHC protocol for M-cadherin antibody 84440-5-RRDownload protocol
IF protocol for M-cadherin antibody 84440-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols