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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of human spleen using 66456-1-Ig (same clone as 66456-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of pig spleen using 66456-1-Ig (same clone as 66456-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of rat spleen using 66456-1-Ig (same clone as 66456-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of RAW 264.7 using 66456-1-Ig (same clone as 66456-1-PBS)

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

Lysozyme Monoclonal antibody, PBS Only

Lysozyme Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66456-1-PBS
Clone No.2G5A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

LYZ, 1,4-beta-N-acetylmuramidase C, 2G5A8, EC:3.2.1.17, Lysozyme C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66456-1-PBS targets Lysozyme in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6917

Product name: Recombinant human Lysozyme protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-148 aa of BC004147

Sequence: CELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV

相同性解析による交差性が予測される生物種
Full Name lysozyme (renal amyloidosis)
Calculated molecular weight 17 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC004147
Gene Symbol Lysozyme
Gene ID (NCBI) 4069
RRIDAB_2881825
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61626
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

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