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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using Lumican Recombinant antibody (85629-1-RR)

    WB analysis of Recombinant protein using 85629-1-RR (same clone as 85629-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Lumican Recombinant antibody (85629-1-RR)

    WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Lumican Recombinant antibody (85629-1-RR)

    WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using Lumican Recombinant antibody (85629-1-RR)

    IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using Lumican Recombinant antibody (85629-1-RR)

    IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

Rat Lumican Recombinant monoclonal antibody, PBS Only

Lumican Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85629-1-PBS
Clone No.242298G6

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Keratan sulfate proteoglycan lumican, KSPG lumican, Lcn, Ldc, Lum

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85629-1-PBS targets Lumican in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3410

Product name: Recombinant Rat Lumican protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 19-338 aa of NM_031050.1

Sequence: QYYDYDAPLFMYGELSPNCAPECNCPHSYPTAMYCDDLKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGKVFSKLKQLKKLHINYNNLTESVGPLPKSLQDLQLANNKISKLGSFDGLVNLTFIYLQHNQLKEEAVSASLKGLKSLEYLDLSFNQMSKLPAGLPTSLLTLYLDNNKITNIPDEYFNRFTGLQYLRLSHNELADSGVPGNSFNISSLLELDLSYNKLKSIPTVNENLENYYLEVNKLEKFDVKSFCKILGPLSYSKIKHLRLDGNPLTQSSLPPDMYECLRVANEITVN

相同性解析による交差性が予測される生物種
Full Name lumican
Calculated molecular weight38 kDa
Observed molecular weight50-90 kDa
GenBank accession numberNM_031050.1
Gene Symbol Lum
Gene ID (NCBI) 81682
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51886
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

umican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

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