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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Livin Polyclonal antibody (27543-1-AP)

    WB analysis using 27543-1-AP (same clone as 27543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Livin Polyclonal antibody (27543-1-AP)

    WB analysis of A431 using 27543-1-AP (same clone as 27543-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using Livin Polyclonal antibody (27543-1-AP)

    WB analysis of A375 using 27543-1-AP (same clone as 27543-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Livin Polyclonal antibody (27543-1-AP)

    IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Livin Polyclonal antibody (27543-1-AP)

    IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

Livin Polyclonal antibody, PBS Only

Livin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27543-1-PBS targets Livin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26737

Product name: Recombinant human BIRC7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-216 aa of BC014475

Sequence: MGPKDSAKCLHRGPQPSHWAAGDGPTQERCGPRSLGSPVLGLDTCRAWDHVDGQILGQLRPLTEEEEEEGAGATLSRGPAFPGMGSEELRLASFYDWPLTAEVPPELLAAAGFFHTGHQDKVRCFFCYGGLQSWKRGDDPWTEHAKWFPSCQFLLRSKGRDFVHSVQETHSQLLGSWDPWEEPEDAAPVAPSVPASGYPELPTPRREVQSESAQEP

相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 7
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC014475
Gene Symbol Livin
Gene ID (NCBI) 79444
RRIDAB_2880901
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CA5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BIRC7 also known as Livin, is one of the family members of the inhibitor of apoptosis protein (IAP). BIRC7 contains a BIR domain that has been shown to bind and inhibit caspases-3, −7 and −9, playing a key role in inhibiting apoptosis. Overexpression of BIRC7 in tumors has been linked with increased chemo- and radio-resistance, recurrence, and decreased patient survival. BIRC7 is closely associated with melanoma disease progression, apoptosis resistance, and chemotherapeutic drug resistance.

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