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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using Lipoprotein(a) Polyclonal antibody (30675-1-AP)

    IHC staining of human placenta using 30675-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30675-1-AP (Lipoprotein(a) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lipoprotein(a) Polyclonal antibody (30675-1-AP)

    IF Staining of HepG2 using 30675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lipoprotein(a) antibody (30675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Lipoprotein(a) Polyclonal antibody

Lipoprotein(a) Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30675-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

LPA, Lp(a), LP, Apolipoprotein(a), APOA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30675-1-AP targets Lipoprotein(a) in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lipoprotein(a) fusion protein Ag32907 相同性解析による交差性が予測される生物種
Full Name lipoprotein, Lp(a)
Calculated molecular weight501 kDa
Observed molecular weight501 kDa
GenBank accession numberNM_005577
Gene Symbol LPA
Gene ID (NCBI) 4018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lipoprotein(a), also known as Lp(a). The plasma concentration of human lipoprotein(a) is correlated with the risk of heart disease. A distinct feature of the Lp(a) particle is the apolipoprotein (a), which is associated with apoB-100, the main protein component of low-density lipoprotein. It is reported that apo(a), which has extensive homology to plasminogen, binds to immobilized fibronectin (PMID: 2531657). The binding of Lp(a) was localized to the C-terminal heparin-binding domain of fibronectin. It is enriched in liver tissue. The N-glycans are complex biantennary structures present in either a mono- or disialylated state.

Protocols

Product Specific Protocols
IHC protocol for Lipoprotein(a) antibody 30675-1-APDownload protocol
IF protocol for Lipoprotein(a) antibody 30675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols