Validation Data Gallery
Tested Applications
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30675-1-AP targets Lipoprotein(a) in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32907 Product name: Recombinant human Lipoprotein(a) protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1835-2040 aa of NM_005577 Sequence: VSLRTRFGKHFCGGTLISPEWVLTAAHCLKKSSRPSSYKVILGAHQEVNLESHVQEIEVSRLFLEPTQADIALLKLSRPAVITDKVMPACLPSPDYMVTARTECYITGWGETQGTFGTGLLKEAQLLVIENEVCNHYKYICAEHLARGTDSCQGDSGGPLVCFEKDKYILQGVTSWGLGCARPNKPGVYARVSRFVTWIEGMMRNN 相同性解析による交差性が予測される生物種 |
| Full Name | lipoprotein, Lp(a) |
| Calculated molecular weight | 501 kDa |
| Observed molecular weight | 501 kDa |
| GenBank accession number | NM_005577 |
| Gene Symbol | LPA |
| Gene ID (NCBI) | 4018 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lipoprotein(a), also known as Lp(a). The plasma concentration of human lipoprotein(a) is correlated with the risk of heart disease. A distinct feature of the Lp(a) particle is the apolipoprotein (a), which is associated with apoB-100, the main protein component of low-density lipoprotein. It is reported that apo(a), which has extensive homology to plasminogen, binds to immobilized fibronectin (PMID: 2531657). The binding of Lp(a) was localized to the C-terminal heparin-binding domain of fibronectin. It is enriched in liver tissue. The N-glycans are complex biantennary structures present in either a mono- or disialylated state.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Lipoprotein(a) antibody 30675-1-AP | Download protocol |
| IHC protocol for Lipoprotein(a) antibody 30675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |