Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31247-1-AP targets Lif in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | leukemia inhibitory factor |
| Calculated molecular weight | 22 KDa |
| Observed molecular weight | 33-35 kDa |
| Gene Symbol | Lif |
| Gene ID (NCBI) | 16878 |
| RRID | AB_3669916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09056 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein containing six putative N-glycosylation sites. The molecular weight of the unglycosylated LIF protein is 20-25 kDa, while the molecular weight of the glycosylated protein is in the range of 37-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Lif antibody 31247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |