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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lamin B2 Recombinant monoclonal antibody (82801-2-RR)

    WB analysis using 82801-2-RR (same clone as 82801-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Lamin B2 Recombinant monoclonal antibody (82801-2-RR)

    WB analysis of A549 using 82801-2-RR (same clone as 82801-2-PBS)

    WB result of Lamin B2 antibody (82801-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected A549 cells. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Lamin B2 Recombinant monoclonal antibody (82801-2-RR)

    IHC staining of human stomach cancer using 82801-2-RR (same clone as 82801-2-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82801-2-RR (Lamin B2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin B2 Recombinant monoclonal antibody (82801-2-RR)

    IF Staining of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Lamin B2 Recombinant monoclonal antibody (82801-2-RR)

    FC experiment of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human Lamin B2 (82801-2-RR, Clone:230184E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82801-2-RR

    Affinity and Kinetic Characterization of 82801-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82801-2-RR against Human Lamin B2 were performed. The affinity constant is 2.81 nM.

  • Featured Product
  • KD/KO Validated

Lamin B2 Recombinant monoclonal antibody, PBS Only

Lamin B2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82801-2-PBS
Clone No.230184E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

LMNB2, 230184E7, LAMB2, Lamin-B2, LMN2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82801-2-PBS targets Lamin B2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1335

Product name: Recombinant human LMNB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 241-600 aa of BC006551

Sequence: EELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM

相同性解析による交差性が予測される生物種
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006551
Gene Symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ03252
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

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